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A photomultiplier steps the emitted chemiluminescence, and outcomes are established through a calibration curve generated by a two-place calibration in addition to a learn curve (5-point calibration) offered by using the reagent barcode.
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cTnI during the sample will become labeled using an ALP-conjugated antibody and is particularly captured onto the sensor floor through the incubation move.[49] Clean fluid made up of enzyme substrate is then applied to clear away unbound substances, and at the same time, ALP bound to the antigen-antibody complicated reacts, releasing an electrochemically detectable solution. The created amperometric sign is instantly proportional on the cTnI within the sample.[50]
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